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Int J Med Sci. 2005;2(2):64-69. Epub 2005 Apr 1.

Cell Cycle Arrest by a Natural Product via G2/M Checkpoint.

Author information

1
Technology Development, CK Life Sciences Int'l Inc., 2 Dai Fu Street, Tai Po Industrial Estate, Hong Kong, China.

Abstract

CKBM is a natural product that exhibits a novel anti-tumor activity through the induction of cell cycle arrest and apoptosis. We have investigated its effects on cell cycle regulation using a gastric cancer cell line, AGS. The effects of CKBM on cell proliferation, cell cycle regulation and apoptosis were analyzed using BrdU (5-bromo-2'-deoxyuridine) cell proliferation assay and flow cytometric analysis, respectively. Specific cellular protein expressions were measured using Western blot analysis. Flow cytometric analysis indicated that CKBM induced G2/M cell cycle arrest and apoptosis, whereas differential protein expressions of p21, p53 and 14-3-3sigma (stratifin) using Western blot analysis were enhanced. The differential expressions of p21, p53 and 14-3-3sigma in AGS cancer cells after CKBM treatment may play critical roles in the G2/M cell cycle arrest that blocks cell proliferation and induces apoptosis.

PMID:
15968342
PMCID:
PMC1145136

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