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Inflamm Res. 2005 May;54(5):204-10.

The C-terminus of murine S100A9 inhibits spreading and phagocytic activity of adherent peritoneal cells.

Author information

1
Laboratory of Pathophysiology, Butantan Institute, Av Vital Brazil, 1500, 05503900 São Paulo, SP, Brazil.

Abstract

OBJECTIVE AND DESIGN:

In the present study, the effect of a synthetic peptide (H(92)-G(102)) identical to the C-terminus of murine S100A9 (mS100A9p) was investigated on adherent peritoneal cell function.

MATERIALS AND METHODS:

For in vitro assays, peritoneal cells were obtained from the abdominal cavity of mice and incubated, with the different concentrations of mS100A9p, for 1 h, and then their spreading and phagocytosis activities were evaluated. For ex-vivo assays, cells obtained from animals treated for 1 h with the peptide were submitted to the mannose-receptor phagocytosis assay. Shorter homologue peptides to the C-terminus of mS100A9p were also evaluated on in vitro phagocytosis assays of Candida albicans particles.

RESULTS:

mS100A9p reduced both the spreading index and phagocytic activity, in vitro and ex-vivo, independent of the receptor evaluated. The homologue peptide corresponding to the H(92)-E(97) region of mS100A9p, the zinc-binding motif, was responsible for such an effect.

CONCLUSION:

These results suggest a modulator effect of the C-terminus of S100A9 protein on the function of adherent peritoneal cells.

PMID:
15953992
DOI:
10.1007/s00011-005-1344-y
[Indexed for MEDLINE]

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