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Int Arch Allergy Immunol. 2005;137 Suppl 1:55-9. Epub 2005 Jun 2.

Role of GATA-3 in IL-5 gene transcription by CD4+ T cells of asthmatic patients.

Author information

1
Department of Immunology, Tokyo Metropolitan Institute of Medical Science, Japan.

Abstract

BACKGROUND:

Helper T cells and T cell cytokines play central roles in allergic disorders including bronchial asthma. We reported enhanced IL-5 production by peripheral blood T cells of asthmatic patients. A transcription factor, GATA-3, has been implicated in IL-5 gene expression. This study was undertaken to clarify the role of GATA-3 in the upregulation of IL-5 synthesis in asthmatic patients.

METHOD:

Peripheral CD4+ T cells were transfected with an IL-5 promoter reporter construct as well as its mutants in the presence or absence of a GATA-3 expression vector. Messenger RNA expression level of GATA-3 in CD4+ T cells of asthmatic subjects was compared to that of healthy donors.

RESULTS:

IL-5 promoter activity in CD4+ T cells was enhanced by overexpression of GATA-3, whereas it was diminished by the introduction of mutations in the putative GATA-3 binding sites. The GATA-3 expression level in CD4+ T cells of asthmatic patients was equivalent to that of healthy controls.

CONCLUSION:

The expression level of GATA-3 may not be an essential factor to cause IL-5 hyperproduction in bronchial asthma, though GATA-3 is crucially involved in IL-5 gene transcription in human peripheral CD4+ T cells.

PMID:
15947486
DOI:
10.1159/000085433
[Indexed for MEDLINE]

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