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J Microbiol Methods. 2005 Nov;63(2):211-5. Epub 2005 Jun 4.

Improvement of the cDNA-AFLP method using fluorescent primers for transcription analysis in bacteria.

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Dipartimento di Biotecnologie Agrarie, Sezione di Microbiologia, Università degli Studi di Firenze, Piazzale delle Cascine 24, I-50144, Firenze, Italy.


Here the cDNA-fluorescent amplified fragment length polymorphism (cDNA-FAFLP) technique, an improvement of cDNA-AFLP method, was used in order to analyse expression profiling in bacteria. The obtained results were validated by real-time PCR. This is the first report that validates the foreseen transcription pattern by cDNA-FAFLP with the application of real-time PCR in bacteria. This new protocol offers the possibility of quick and reliable analysis of transcription profiles, also avoids the problem linked with the use of radioisotopes, and allows a quick identification of genes differentially expressed in bacteria.

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