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EMBO J. 2005 Jun 15;24(12):2172-82. Epub 2005 Jun 2.

PIASy mediates SUMO-2 conjugation of Topoisomerase-II on mitotic chromosomes.

Author information

1
Laboratory of Gene Regulation and Development, NICHD, NIH, Bethesda, MD 20892-5431, USA.

Abstract

Here we show that the PIASy protein is specifically required for mitotic modification of Topoisomerase-II by SUMO-2 conjugation in Xenopus egg extracts. PIASy was unique among the PIAS family members in its capacity to bind mitotic chromosomes and recruit Ubc9 onto chromatin. These properties were essential, since PIASy mutants that did not bind chromatin or failed to recruit Ubc9 were functionally inactive. We observed that PIASy depletion eliminated essentially all chromosomal accumulation of EGFP-SUMO-2-conjugated species, suggesting that it is the primary E3-like factor for mitotic chromosomal substrates of SUMO-2. PIASy-dependent SUMO-2-conjugated species were highly concentrated on the inner centromere, and inhibition of PIASy blocked anaphase sister chromatid segregation in egg extracts. Taken together, our observations suggest that PIASy is a critical regulator of mitotic SUMO-2 conjugation for Topoisomerase-II and other chromosomal substrates, and that its activity may have particular relevance for centromeric functions required for proper chromosome segregation.

PMID:
15933717
PMCID:
PMC1150894
DOI:
10.1038/sj.emboj.7600700
[Indexed for MEDLINE]
Free PMC Article

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