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Appl Environ Microbiol. 2005 Jun;71(6):3342-7.

Genomic DNA amplification from a single bacterium.

Author information

1
Molecular Staging, Inc., New Haven, Connecticut, USA.

Abstract

Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using phi 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying non-culturable microorganisms.

PMID:
15933038
PMCID:
PMC1151817
DOI:
10.1128/AEM.71.6.3342-3347.2005
[Indexed for MEDLINE]
Free PMC Article
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