Format

Send to

Choose Destination
J Forensic Sci. 2005 May;50(3):548-54.

Forensic casework analysis using the HVI/HVII mtDNA linear array assay.

Author information

1
Uppsala University, Department of Genetics and Pathology, Rudbeck Laboratory 751 85 Uppsala, Sweden.

Abstract

The mitochondrial hypervariable regions I and II have proven to be a useful target for analysis of forensic materials, in which the amount of DNA is limited or highly degraded. Conventional mitochondrial DNA (mtDNA) sequencing can be time-consuming and expensive, limitations that can be minimized using a faster and less expensive typing assay. We have evaluated the exclusion capacity of the linear array mtDNA HVI/HVII region-sequence typing assay (Roche Applied Science) in 16 forensic cases comprising 90 samples. Using the HVI/HVII mtDNA linear array, 56% of the samples were excluded and thus less than half of the samples require further sequencing due to a match or inconclusive results. Of all the samples that were excluded by sequence analysis, 79% could be excluded using the HVI/HVII linear array alone. Using the HVI/HVII mtDNA linear array assay, we demonstrate the potential to decrease sequencing efforts substantially and thereby reduce the cost and the turn-around time in casework analysis.

PMID:
15932085
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center