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Am J Physiol Cell Physiol. 2005 Oct;289(4):C868-80. Epub 2005 Jun 1.

Expression and characterization of delayed rectifying K+ channels in anterior rat taste buds.

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  • 1Department of Biology and The Center for Integrated BioSystems, Utah State University, 5305 Old Main Hill, Logan, Utah 84322-5305, USA.

Abstract

Delayed rectifying K+ (DRK) channels in taste cells have been implicated in the regulation of cell excitability and as potential targets for direct and indirect modulation by taste stimuli. In the present study, we have used patch-clamp recording to determine the biophysical properties and pharmacological sensitivity of DRK channels in isolated rat fungiform taste buds. Molecular biological assays at the taste bud and single-cell levels are consistent with the interpretation that taste cells express a variety of DRK channels, including members from each of the three major subfamilies: KCNA, KCNB, and KCNC. Real-time PCR assays were used to quantify expression of the nine DRK channel subtypes. While taste cells express a number of DRK channels, the electrophysiological and molecular biological assays indicate that the Shaker Kv1.5 channel (KCNA5) is the major functional DRK channel expressed in the anterior rat tongue.

PMID:
15930148
DOI:
10.1152/ajpcell.00115.2005
[PubMed - indexed for MEDLINE]
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