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Biochem Biophys Res Commun. 2005 Jul 8;332(3):633-9.

Gene expression using an ultrathin needle enabling accurate displacement and low invasiveness.

Author information

1
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974, Japan.

Abstract

We have previously demonstrated a new cell manipulation technology by using an atomic force microscope (AFM) and ultrathin needles, named nanoneedles. The nanoneedle is an AFM tip etched by a focused ion beam (FIB) and is sharpened from 200 to 800 nm in diameter. In this study, we have evaluated the proper diameter of a needle required for insertion into human cells over a long period without causing cell death, and achieved highly efficient gene expression method for human cells using a nanoneedle and an AFM.

PMID:
15925564
DOI:
10.1016/j.bbrc.2005.04.059
[Indexed for MEDLINE]

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