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Clin Chim Acta. 2005 Jul 24;357(2):210-8.

Analytical assessment of MALDI-TOF Imaging Mass Spectrometry on thin histological samples. An insight in proteome investigation.

Author information

1
Centro Studi sull'Invecchiamento (Ce.S.I.), Fondazione UniversitĂ  "G.D'Annunzio", Chieti, Italy.

Abstract

BACKGROUND:

The development of MALDI Imaging Mass Spectrometry is a promising technique in the investigation of biological molecular repertoire. We have pursued an analytical assessment of this technique in its application to proteome analysis.

METHODS:

A specific statistical method of analysis has been developed to enable data processing in the absence of internal standards, by defining similarity scores.

RESULTS:

The investigated linear mode MALDI-TOF set-up allows to obtain data variations comprised within the 30% of variation when assaying tissues samples from the same animal, while the 60% of variation was highlighted in the inter-mice series assaying syngenic animals.

CONCLUSIONS:

This analytical assessment represents the first step of a process that should validate the utilisation of this technique in the clinical practice.

PMID:
15913587
DOI:
10.1016/j.cccn.2005.03.029
[Indexed for MEDLINE]

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