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Anal Biochem. 2005 Jul 15;342(2):312-7.

Selection of RNA aptamers against human influenza virus hemagglutinin using surface plasmon resonance.

Author information

1
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology,Tsukuba City 305-8566, Japan.

Abstract

Aptamers are functional nucleic acids possessing high affinity and specificity to their cognate ligands and are isolated from a library of nucleic acids by iterative rounds of selection and amplification. In the current study, we used surface plasmon resonance (Biacore) as an efficient methodology for selecting aptamers that bind to hemagglutinin (HA) of human influenza virus. This procedure allowed us to monitor and select the target-bound aptamers specifically and simultaneously. These studies not only yielded an aptamer that binds to the HA of influenza virus with high affinity but also revealed the consensus sequence, 5'-GUCGNCNU(N)(2-3)GUA-3, for HA recognition.

PMID:
15913532
DOI:
10.1016/j.ab.2005.04.013
[Indexed for MEDLINE]
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