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Diabetologia. 2005 Jul;48(7):1411-5. Epub 2005 May 21.

Expression of 14-3-3 zeta and interaction with protein kinase C in the rat retina in early diabetes.

Author information

1
Department of Anatomy and Neurobiology, College of Medicine, Institute of Health Science, Gyeongsang National University, Jinju, South Korea.

Abstract

AIMS/HYPOTHESIS:

The present study aimed to investigate the expression levels of and the relationship between 14-3-3 zeta and protein kinase C (PKC) in the retina of early diabetes.

METHODS:

Changes in the expression levels of, and interaction between, 14-3-3 zeta and PKC were investigated by Northern and Western blot analyses, immunoprecipitation and double immunostaining in the retina of diabetic rats after 6 weeks of diabetes. PKC activity was examined using a PKC assay.

RESULTS:

In the diabetic retina, the molecular levels of 14-3-3 zeta were reduced, while those of PKC beta and zeta were increased. Direct interaction between 14-3-3 zeta and PKC was markedly decreased in the retina after 6 weeks of diabetes, while PKC activity was increased.

CONCLUSIONS/INTERPRETATION:

These findings show that a reduction in 14-3-3 zeta can induce PKC activation, suggesting that this is a main cause of visual dysfunction in the retina during diabetes.

PMID:
15909155
DOI:
10.1007/s00125-005-1774-7
[Indexed for MEDLINE]

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