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Nat Cell Biol. 2005 Jun;7(6):633-6. Epub 2005 May 22.

Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies.

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Department of Molecular Pharmacology, 269 Campus Drive, CCSR 4225A, Stanford University School of Medicine, Stanford, CA 94305, USA.


RNA interference (RNAi) is an important means of eliminating mRNAs, but the intracellular location of RNA-induced silencing complex (RISC) remains unknown. We show here that Argonaute 2, a key component of RISC, is not randomly distributed but concentrates in mRNA decay centres that are known as cytoplasmic bodies. The localization of Argonaute 2 in decay centres is not altered by the presence or absence of small interfering RNAs or their targeted mRNAs. However, RNA is required for the integrity of cytoplasmic bodies because RNase eliminates Argonaute 2 localization. In addition, Argonaute 1, another Argonaute family member, is concentrated in cytoplasmic bodies. These results provide new insight into the mechanism of RNAi function.

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