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J Bacteriol. 2005 Jun;187(11):3869-72.

Alginate lyase (AlgL) activity is required for alginate biosynthesis in Pseudomonas aeruginosa.

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1
Division of Biomedical Sciences, University of California, Riverside, Riverside, California 92521-0121, USA.

Abstract

To determine whether AlgL's lyase activity is required for alginate production in Pseudomonas aeruginosa, an algLdelta::Gm(r) mutant (FRD-MA7) was created. algL complementation of FRD-MA7 restored alginate production, but algL constructs containing mutations inactivating lyase activity did not, demonstrating that the enzymatic activity of AlgL is required for alginate production.

PMID:
15901714
PMCID:
PMC1112040
DOI:
10.1128/JB.187.11.3869-3872.2005
[Indexed for MEDLINE]
Free PMC Article
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