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Am J Vet Res. 2005 Apr;66(4):721-6.

A critical role of interleukin-10 in the response of bovine macrophages to infection by Mycobacterium avium subsp paratuberculosis.

Author information

1
Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108, USA.

Abstract

OBJECTIVES:

To evaluate the role of interleukin (IL)-10 in the inability of monocyte-derived bovine macrophages to kill Mycobacterium avium subsp paratuberculosis organisms in vitro.

SAMPLE POPULATION:

Monocytes were obtained from healthy adult Holstein dairy cows that had negative results when tested for infection with M avium subsp paratuberculosis.

PROCEDURE:

Monocyte-derived macrophages were incubated with M avium subsp paratuberculosis for 2, 6, 24, 72, or 96 hours with or without addition of saturating concentrations of a goat anti-human IL-10 that has been documented to neutralize bovine IL-10 activity. Variables assessed included ingestion and killing of M avium subsp paratuberculosis; expression of tumor necrosis factor (TNF)-alpha, IL-12, IL-8, major histocompatability (MHC) class II, vacuolar H+ ATPase, and B cell CLL/lymphoma 2 (BCL-2); production of nitric oxide; acidification of phagosomes; and apoptosis of macrophages.

RESULTS:

Neutralization of IL-10 enabled macrophages to kill 57% of M avium subsp paratuberculosis organisms within 96 hours. It also resulted in an increase in expression of TNF-alpha, IL-12, IL-8, MHC class II, and vacuolar H+ ATPase; decrease in expression of BCL-2; increase in acidification of phagosomes; apoptosis of macrophages; and production of nitric oxide.

CONCLUSIONS AND CLINICAL RELEVANCE:

The capacity of M avium subsp paratuberculosis to induce IL-10 expression may be a major determinant of virulence for this organism.

PMID:
15900955
DOI:
10.2460/ajvr.2005.66.721
[Indexed for MEDLINE]

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