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J Microbiol Methods. 2005 Oct;63(1):95-8.

An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens.

Author information

1
Department of Medical Microbiology, University of Aberdeen, U.K.

Abstract

A single-step, 5-min lysis method was investigated as a rapid technique to extract genomic DNA from mycobacteria for PCR detection of M. tuberculosis directly from clinical specimens. Of 67 smear-positive clinical specimens, 64 (95.5%) were positive by PCR after this rapid extraction method.

PMID:
15893828
DOI:
10.1016/j.mimet.2005.03.005
[Indexed for MEDLINE]

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