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Neurosci Res. 2005 Jun;52(2):132-8. Epub 2005 Mar 17.

Large-scale imaging of cortical network activity with calcium indicators.

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1
Department of Biological Sciences, Columbia University, 1002 Fairchild Center, M.C. 2435 New York, NY 10027, USA. ikegaya@tk.air.jp

Abstract

Bulk loading of calcium indicators has provided a unique opportunity to reconstruct the activity of cortical networks with single-cell resolution. Here we describe the detailed methods of bulk loading of AM dyes we developed and have been improving for imaging with a spinning disk confocal microscope.

PMID:
15893573
DOI:
10.1016/j.neures.2005.02.004
[Indexed for MEDLINE]
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