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Chemphyschem. 2005 May;6(5):881-8.

Proteins in action monitored by time-resolved FTIR spectroscopy.

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Lehrstuhl für Biophysik, ND 04/596, Ruhr-Universität Bochum, 44801 Bochum, Germany.


In the post genome era proteins coming into the focus of life sciences. X-ray structure analysis and NMR spectroscopy are established methods to determine the geometry of proteins. In order to determine the molecular reaction mechanism of proteins, time-resolved FTIR (trFTIR) difference spectroscopy emerges as a valuable tool. In this Minireview we describe the trFTIR difference spectroscopy and show its application on the light-driven proton pump bacteriorhodopsin (bR), the photosynthetic reaction center and the GTPase Ras, which is crucial in signal transduction. The main principles of the technique are presented, including a summary of triggering techniques, scan modes and analysis.

[Indexed for MEDLINE]

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