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Am J Respir Crit Care Med. 2005 Aug 1;172(3):314-21. Epub 2005 May 5.

House dust mite facilitates ovalbumin-specific allergic sensitization and airway inflammation.

Author information

1
Division of Respiratory Diseases and Allergy, Center for Gene Therapeutics, McMaster University, Hamilton, Ontario, Canada.

Abstract

RATIONALE:

Mouse models of allergic airway disease have greatly contributed to our understanding of disease induction and pathogenesis. Although these models typically investigate responses to a single antigen or allergen, humans are frequently exposed to a myriad of allergens, each with distinct antigenic potential.

OBJECTIVES:

Given that airway exposure to ovalbumin (OVA), a prototypic innocuous antigen, induces inhalation tolerance, we wished to investigate how this response would be altered if OVA were encountered concurrently with a house dust mite extract (HDM), which we have recently shown is capable of eliciting a robust allergic airway inflammatory response that is mediated, at least in part, by granulocyte-macrophage colony-stimulating factor.

METHODS:

Balb/c mice were exposed daily to HDM (intranasally) followed immediately by exposure to aerosolized OVA for 5 weeks. To allow the inflammatory response elicited by HDM to subside fully, mice were then allowed to rest, unexposed, for 8 weeks, at which time they were rechallenged with aerosolized OVA for 3 consecutive days.

MEASUREMENTS AND MAIN RESULTS:

At this time, we observed a robust eosinophilic inflammatory response in the lung that was associated with an increase in bronchial hyperreactivity. Moreover, we documented significantly elevated serum levels of OVA-specific IgE and IgG(1) and increased production of the Th2 cytokines interleukin 4 (IL-4), IL-5, and IL-13 by splenocytes stimulated in vitro with OVA.

CONCLUSION:

Our data demonstrate the potential of a potent allergen such as HDM to establish a lung microenvironment that fosters the development of allergic sensitization to otherwise weak or innocuous antigens, such as OVA.

PMID:
15879422
DOI:
10.1164/rccm.200502-198OC
[Indexed for MEDLINE]

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