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Biomacromolecules. 2005 May-Jun;6(3):1602-7.

Protein-functionalized polymer brushes.

Author information

1
Laboratoire des Polymères, Institut des Matériaux, Bâtiment MX-D, and Laboratoire d'Ingénierie des Protéines, Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.

Abstract

A new strategy for the preparation of protein-functionalized polymer brushes is reported, which is based on a combination of surface-initiated atom transfer radical polymerization (ATRP), p-nitrophenyl chloroformate activation of the surface hydroxyl groups, and subsequent O(6)-benzylguanine (BG) functionalization. The BG-functionalized brushes are used to chemoselectively immobilize O(6)-alkylguanine-DNA-alkyltransferase (AGT) fusion proteins with a defined orientation and surface density. These protein-modified polymer brushes are attractive candidates for the development of protein microarrays.

PMID:
15877383
DOI:
10.1021/bm050016n
[Indexed for MEDLINE]

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