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Clin Nutr. 2005 Jun;24(3):360-6.

Cardioprotective effects of Ilex paraguariensis extract: evidence for a nitric oxide-dependent mechanism.

Author information

1
Cátedra de Farmacología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, CIC, La Plata 1900, Buenos Aires, Argentina.

Abstract

AIM:

To examine the effects of an Ilex paraguariensis (Ip) extract on postischemic alterations derived from 20 min of global ischemia and 30 min of reperfusion.

METHODS:

Isolated rat hearts were treated 10 min before ischemia and the first 10 min of reperfusion with Ip 30 microg/ml. In other hearts, chelerythrine (1 microM), a protein kinase C blocker, or l(G)-nitro l-arginine methyl ester (l-NAME), a nitric oxide synthase inhibitor, were administered prior to Ip infusion. Left ventricular developed pressure (LVDP), +dP/dt(max), -dP/dt(max), and left ventricular end diastolic pressure (LVEDP) were used to assess myocardial function. Thiobarbituric acid reactive substances (TBARS) were measured.

RESULTS:

Ip treatment produced an improvement of postichemic recovery (LVDP=96+/-8%; +dP/dt(max)=95+/-10%; -dP/dt(max)=90+/-12% vs. 57+/-6%, 53+/-6% and 57+/-8%, respectively, in untreated hearts) and an attenuation of the increase of LVEDP and TBARS content. Chelerythrine did not modify and l-NAME abolished the protection induced by Ip.

CONCLUSIONS:

These data are the first demonstration that Ip extract attenuates the myocardial dysfunction provoked by ischemia and reperfusion and that this cardioprotection involves a diminution of oxidative damage through a nitric oxide-dependent mechanism.

PMID:
15869828
DOI:
10.1016/j.clnu.2004.11.013
[Indexed for MEDLINE]

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