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Fertil Steril. 2005 May;83(5):1442-6.

Effects of magnetic-activated cell sorting on sperm motility and cryosurvival rates.

Author information

1
Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute, Cleveland, Ohio 44195, USA.

Abstract

OBJECTIVE:

To evaluate the effect of magnetic-activated cell sorting in cryopreservation-thawing protocols on sperm motility and cryosurvival rate.

DESIGN:

Prospective-controlled study.

SETTING:

Andrology department at a university-based medical institution.

PATIENT(S):

Ten healthy volunteer sperm donors.

INTERVENTION(S):

Sperm populations were separated using annexin-V magnetic-activated cell sorting before and after the cryopreservation-thawing process.

MAIN OUTCOME MEASURE(S):

Sperm motility and cryosurvival rate.

RESULT(S):

Annexin-negative sperm separated by magnetic-activated cell sorting had statistically significantly higher motility following cryopreservation-thawing than sperm that were not separated. Similarly, annexin-negative spermatozoa also had higher cryosurvival rate than sperm cryopreserved without magnetic-activated cell sorting and sperm that were annexin-positive.

CONCLUSION(S):

Superparamagnetic annexin V-conjugated microbeads can separate spermatozoa with externalized phosphatidylserine, which is considered one of the early features of late apoptosis. The separation of a distinctive population of nonapoptotic spermatozoa with intact membranes may optimize the cryopreservation-thawing outcome. Magnetic-activated cell sorting using annexin-V microbeads enhances sperm motility and cryosurvival rates following cryopreservation.

[Indexed for MEDLINE]

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