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Bioorg Med Chem Lett. 2005 May 16;15(10):2447-51.

Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing.

Author information

1
Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.

Abstract

One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. Herein, we report a bacterium-based, intein-mediated strategy to generate N-terminal cysteine-containing proteins which are then chemoselectively immobilized to a thioester-functionalized glass slide to generate the corresponding protein microarray. We also showed preliminary data of the strategy in a yeast host system.

PMID:
15863295
DOI:
10.1016/j.bmcl.2005.03.079
[Indexed for MEDLINE]

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