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Nat Struct Mol Biol. 2005 May;12(5):469-70. Epub 2005 Apr 24.

Target accessibility dictates the potency of human RISC.

Author information

1
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.

Abstract

In this report, we examined the effect of increased target site access on activated human RNA-induced silencing complex (RISC(*)) catalysis. Kinetic studies revealed that siRNA-programmed RISC(*) cleaved target RNA with higher efficiencies when target site access was increased. These results provide evidence that target site access is linked to RISC(*) catalysis.

PMID:
15852021
DOI:
10.1038/nsmb931
[Indexed for MEDLINE]

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