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Proc Natl Acad Sci U S A. 2005 Apr 26;102(17):5905-8. Epub 2005 Apr 19.

How restriction enzymes became the workhorses of molecular biology.

Author information

1
New England Biolabs, 32 Tozer Road, Beverly, MA 01915, USA. roberts@neb.com

Abstract

Restriction enzymes have proved to be invaluable for the physical mapping of DNA. They offer unparalleled opportunities for diagnosing DNA sequence content and are used in fields as disparate as criminal forensics and basic research. In fact, without restriction enzymes, the biotechnology industry would certainly not have flourished as it has. The first experiments demonstrating the utility of restriction enzymes were carried out by Danna and Nathans and reported in 1971. This pioneering study set the stage for the modern practice of molecular biology in which restriction enzymes are ubiquitous tools, although they are often taken for granted.

PMID:
15840723
PMCID:
PMC1087929
DOI:
10.1073/pnas.0500923102
[Indexed for MEDLINE]
Free PMC Article

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