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FEMS Microbiol Lett. 2005 Apr 15;245(2):329-36.

The Listeria monocytogenes prfAP2 promoter is regulated by sigma B in a growth phase dependent manner.

Author information

1
Department of Food Science, Cornell University, 415 Stocking Hall, Ithaca, NY 14853, USA. ues3@cornell.edu

Abstract

Listeria monocytogenes prfA, encoding positive regulatory factor A, is transcribed from three promoters (prfAP1, prfAP2, and PplcA). The prfAP2 promoter was previously proposed to be sigma B (sigma(B))-dependent. This hypothesis was tested by creating prfA promoter-gus transcriptional fusions in both L. monocytogenes wild-type (wt) and DeltasigB backgrounds and then measuring (i) beta-glucuronidase (GUS) activities; (ii) gus mRNA transcript levels; and (iii) the presence or absence of GUS in cells by immunofluorescence staining. prfAP2-directed expression increased as the wt L. monocytogenes strain entered stationary phase, whereas prfAP2-directed expression was greatly reduced in the DeltasigB strain, confirming both growth phase- and sigma(B)-dependent transcription of prfAP2. We conclude that prfAP2 is directly regulated by sigma(B).

PMID:
15837390
DOI:
10.1016/j.femsle.2005.03.025
[Indexed for MEDLINE]
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