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BMC Cancer. 2005 Apr 18;5:41.

Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa.

Author information

1
Institute of Medical Technology, University of Tampere, and Tampere University Hospital, Tampere, Finland. laura.kummola@uta.fi

Abstract

BACKGROUND:

Carbonic anhydrase (CA) isozymes may have an important role in cancer development. Some isozymes control pH homeostasis in tumors that appears to modulate the behaviour of cancer cells. CA XIII is the newest member of the CA gene family. It is a cytosolic isozyme which is expressed in a number of normal tissues. The present study was designed to investigate CA XIII expression in prospectively collected colorectal tumor samples.

METHODS:

Both neoplastic and normal tissue specimens were obtained from the same patients. The analyses were performed using CA XIII-specific antibodies and an immunohistochemical staining method. For comparison, the tissue sections were immunostained for other cytosolic isozymes, CA I and II.

RESULTS:

The results indicated that the expression of CA XIII is down-regulated in tumor cells compared to the normal tissue. The lowest signal was detected in carcinoma samples. This pattern of expression was quite parallel for CA I and II.

CONCLUSION:

The down-regulation of cytosolic CA I, II and XIII in colorectal cancer may result from reduced levels of a common transcription factor or loss of closely linked CA1, CA2 and CA13 alleles on chromosome 8. Their possible role as tumor suppressors should be further evaluated.

PMID:
15836783
PMCID:
PMC1097719
DOI:
10.1186/1471-2407-5-41
[Indexed for MEDLINE]
Free PMC Article

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