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J Microsc. 1992 Mar;165(Pt 3):417-26.

The double disector: unbiased stereological estimation of the number of particles inside other particles.

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University Institute of Pathology, University of Aarhus, Denmark.


A double-disector method for obtaining estimates of the number of particles inside other particles, illustrated by an estimation of the average cell number in a glomerulus, has been developed. The method is suitable for paraffin-embedded tissue because it does not require knowledge about the section thickness. Most importantly, the estimate is absolutely unaffected by tissue shrinkage. The average number of cells in a human glomerulus is 2850 whereas in rats it is 580.

[Indexed for MEDLINE]

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