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Proteomics. 2005 May;5(8):2010-7.

Peptide microarrays for the detection of molecular interactions in cellular signal transduction.

Author information

1
Institute for Cell Biology, University of Tübingen, Germany.

Abstract

The formation of protein complexes is a hallmark of cellular signal transduction. Here, we show that peptide microarrays provide a robust and quantitative means to detect signalling-dependent changes of molecular interactions. Recruitment of a protein into a complex upon stimulation of a cell leads to the masking of an otherwise exposed binding site. In cell lysates this masking can be detected by reduced binding to a microarray carrying a peptide that corresponds to the binding motif of the respective interaction domain. The method is exemplified for the lymphocyte-specific tyrosine kinase 70 kDa zeta-associated protein binding to a bis-phosphotyrosine-motif of the activated T-cell receptor via its tandem SH2 domain. Compared to established techniques, the method provides a significant shortcut to the detection of molecular interactions.

PMID:
15832366
DOI:
10.1002/pmic.200401095
[Indexed for MEDLINE]

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