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J Am Acad Dermatol. 1992 May;26(5 Pt 1):744-8.

Distribution patterns and frequency of proliferating cells in cutaneous keratinocytic neoplasms. Immunohistochemical study with a monoclonal antibody (TOB7) used against proliferating cell nuclear antigen.

Author information

1
Department of Dermatology, Shinshu University School of Medicine, Matsumoto, Japan.

Abstract

BACKGROUND:

Almost all markers for proliferating cells need freshly frozen tissues for evaluation; therefore retrospective study is impossible.

OBJECTIVE:

In the present study, a murine monoclonal antibody (TOB7) against the proliferating cell nuclear antigen (PCNA) was used for the analysis of cell kinetics of cutaneous keratinocytic neoplasms. The antibody is applicable to formalin-fixed, paraffin-embedded tissues.

METHODS:

The frequency of PCNA-positive cells and their distribution patterns were immunohistochemically investigated in various cutaneous keratinocytic neoplasms.

RESULTS:

Squamous cell carcinoma and Bowen's disease showed significantly increased numbers of PCNA-positive cells when compared with other keratinocytic neoplasms. A characteristic marginal or random distribution pattern of PCNA-positive cells was observed in the lesions of each disease category.

CONCLUSION:

Important information on the growth dynamics of keratinocytic neoplasms was obtained in this retrospective immunohistochemical study.

PMID:
1583174
DOI:
10.1016/0190-9622(92)70104-n
[Indexed for MEDLINE]

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