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Biophys Chem. 2005 Apr 22;114(2-3):199-204. Epub 2004 Dec 22.

Lysozyme effect on structural state of model membranes as revealed by pyrene excimerization studies.

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  • 1Department of Biological and Medical Physics, V.N. Karazin Kharkov National University, Ukraine.


Steady-state measurements of pyrene fluorescence in the model bilayer membranes composed of phosphatidylcholine (PC) and its mixtures with cardiolipin (CL) have been performed to gain insight into the effect of lysozyme on molecular organization of lipid bilayer. Analysis of vibronic structure of the probe emission spectra revealed no changes in transverse distribution of pyrene monomers on varying CL contents or increasing the extent of lysozyme binding to liposomes. Excimer-to-monomer fluorescence intensity ratio has been found to reduce on lysozyme association with lipids. The magnitude of this effect increased with increasing CL content from 0 to 40 mol%. These results have been interpreted as indicating decrease in the membrane free volume on formation of both electrostatic and hydrophobic protein-lipid contacts.

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