Format

Send to

Choose Destination
Int Arch Allergy Immunol. 1992;97(1):17-24.

The potent IgG4-inducing antigen in banana is a mannose-binding lectin, BanLec-I.

Author information

1
Central Laboratory, The Netherlands' Red Cross Blood Transfusion Service, Amsterdam.

Abstract

IgG4 antibodies to banana were found to occur far more frequently than expected. The most important antigen involved proved to be a lectin, BanLec-I. Because of the lectin nature of the antigen, it was important to establish the antibody nature of the lectin-IgG4 interaction and to exclude an interaction between the sugar-binding site of the lectin and glycosidic chains on IgG4. Three arguments in support of immune binding are: (1) the binding of BanLec-I to IgG4 is mannoside resistant, whereas the binding to all other glycoproteins tested is mannoside inhibitable; (2) only a minor fraction of the IgG4 in serum and none of five IgG4 myelomas tested was bound, and (3) the lectin binds to the Fab fragment of the IgG4 molecule. A curious finding was that in the presence of high-molecular-weight glycoproteins the interaction between IgG4 and BanLec-I was enhanced by alpha-methyl mannoside. The probable explanation of this phenomenon is that complexes of the lectin with high-molecular-weight glycoproteins by sterical interference inhibit the interaction with human IgG4 antibodies (or with rabbit antibodies to the lectin). This inhibition is prevented in the presence of alpha-methyl mannoside. These results support the earlier suggestion that some lectins are particularly prone to induce an immune response upon oral feeding. This banana lectin might be a potentially useful carrier protein for oral antihapten immunization in humans.

PMID:
1582693
DOI:
10.1159/000236090
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for S. Karger AG, Basel, Switzerland
Loading ...
Support Center