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J Microbiol Methods. 2005 Jul;62(1):125-7.

Rapid protocol for electroporation of Clostridium perfringens.

Author information

1
Institute of Microbiology AS CR, Vídenská 1083, 14220 Prague 4, Czech Republic.

Abstract

A rapid and simple method has been developed for the electroporation of Clostridium perfringens with plasmid DNA. The new improvements, harvesting cells early in the logarithmic stage of growth, keeping the cells at room temperature and the absence of post-shock incubation on ice increased transformation efficiency by one order of magnitude.

PMID:
15823401
DOI:
10.1016/j.mimet.2005.01.012
[Indexed for MEDLINE]

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