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Circ Shock. 1992 Feb;36(2):140-6.

Dietary enrichment with omega-3 fatty acids partially protects against lipopolysaccharide-induced atrial depression in rats.

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Department of Anesthesiology, University of Massachusetts Medical Center, Worcester 01655.


We tested the hypothesis that pretreatment with a diet enriched with omega-3 fatty acids can prevent lipopolysaccharide (LPS)-induced atrial dysfunction. Sprague-Dawley rats were fed a diet containing 20% safflower oil (control diet; CD) or 19.5% menhaden/0.5% safflower oil (experimental diet; ED). After 28 days, the animals were injected I.V. with LPS (20 mg/kg) or normal saline (S). Two hours later, the atria were harvested, connected to a force displacement transducer-amplifier-recorder system and maintained in vitro in oxygenated 37.5 degrees C Krebs-Henseleit buffer. Force of contraction indexed to body weight (FOCI; g/kg) and maximal rate of rise of contraction (dF/dt, g/sec) and relaxation (-dF/dt, g/sec) were similar in the CD-S (n = 6) and ED-S (n = 6) groups. FOCI, dF/dt, and -dF/dt were lower (P less than 0.05) in rats injected with LPS compared with rats injected with S irrespective of diet, but were significantly higher (P less than 0.05) in LPS-ED rats (n = 11) compared with LPS-CD rats (n = 11). Chronotropic and inotropic responses to graded doses (0.1, 0.5, 1.0, and 5.0 microM) of isoproterenol were not significantly different among groups. LPS-induced production of thromboxane B2 but not 6-keto-prostaglandin F1 alpha, was inhibited in ED fed rats. The ED did not enhance survival when rats were challenged with a 20 mg/kg I.V. dose of LPS. These results indicate that dietary enrichment with omega-3 fatty acids in rats partially protects against LPS-induced alterations in atrial function but does not change mortality after an LD100 dose of LPS.

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