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Methods Enzymol. 2005;393:269-88.

Real-time reporting of circadian-regulated gene expression by luciferase imaging in plants and mammalian cells.

Author information

1
Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA.

Abstract

Luciferase enzymes have been used as reporters of circadian rhythms in organisms as diverse as cyanobacteria, plants, fruit flies, and mice. This article details methodology for real-time reporting of circadian-regulated gene expression by imaging of luciferase bioluminescence in plants and mammalian cells.

PMID:
15817294
DOI:
10.1016/S0076-6879(05)93011-5
[Indexed for MEDLINE]

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