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Anticancer Res. 2005 Jan-Feb;25(1A):363-8.

Induction of apoptosis in human promyelocytic leukemia cells by a natural trachylobane diterpene.

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1
Laboratoire de Pharmacognosie, Université Catholique de Louvain, UCL 72.30-CHAM, 1200 Bruxelles, Belgium.

Abstract

BACKGROUND:

Trachylobane diterpenes are secondary metabolites, quite rare in nature, and their bioactivities are poorly understood. Recently, we have described the cytotoxic activity of ent-trachyloban-3beta-ol isolated from the leaves of Croton zambesicus, a plant used in African folk medicine.

MATERIALS AND METHODS:

Cell viability on several cell lines, cell morphology, DNA laddering, annexin Vand caspase-3 activation experiments were undertaken in order to analyse the cytotoxicty of trachylobane diterpene and to determine if this compound is able to induce apoptosis.

RESULTS:

ent-Trachyloban-3beta-ol exerts a dose-dependent cytotoxic effect, which varies between cell lines. Induction of apoptosis in HL-60 cells could be detected at a concentration of 50 microM after 24-h treatment.

CONCLUSION:

We show here, for the first time, that a trachylobane diterpene is able to induce apoptosis in human promyelocytic leukemia cells via caspase-3 activation in a concentration-dependent manner.

PMID:
15816559
[Indexed for MEDLINE]
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