Format

Send to

Choose Destination
See comment in PubMed Commons below
Curr Opin Chem Biol. 2005 Apr;9(2):210-6.

High-throughput screens and selections of enzyme-encoding genes.

Author information

  • 1Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel.

Abstract

The availability of vast gene repertoires from both natural sources (genomic and cDNA libraries) and artificial sources (gene libraries) demands the development and application of novel technologies that enable the screening or selection of large libraries for a variety of enzymatic activities. We describe recent developments in the selection of enzyme-coding genes for directed evolution and functional genomics. We focus on HTS approaches that enable selection from large libraries (>10(6) gene variants) with relatively humble means (i.e. non-robotic systems), and on in vitro compartmentalization in particular.

PMID:
15811807
DOI:
10.1016/j.cbpa.2005.02.002
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center