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Int J Biol Macromol. 2005 Apr;35(3-4):163-7.

Immobilization of tyrosinase in polysiloxane/polypyrrole copolymer matrices.

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1
Department of Chemistry, Middle East Technical University, 06531 Ankara, Turkey.

Abstract

Immobilization of tyrosinase in conducting copolymer matrices of pyrrole functionalized polydimethylsiloxane/polypyrrole (PDMS/PPy) was achieved by electrochemical polymerization. The polysiloxane/polypyrrole/tyrosinase electrode was constructed by the entrapment of enzyme in conducting matrices during electrochemical copolymerization. Maximum reaction rate (V(max)) and Michaelis-Menten constant (K(m)) were investigated for immobilized enzyme. Enzyme electrodes were prepared in two different electrolyte/solvent systems. The effect of supporting electrolytes, p-toluene sulfonic acid and sodium dodecyl sulfate on the enzyme activity and film morphology were determined. Temperature and pH optimization, operational stability and shelf-life of enzyme electrodes were also examined. Phenolic contents of green and black tea were determined by using enzyme electrodes.

PMID:
15811471
DOI:
10.1016/j.ijbiomac.2005.01.006
[Indexed for MEDLINE]
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