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Transplant Proc. 2005 Jan-Feb;37(1):67-9.

Efficiency of adenovirus-mediated gene transduction in heart grafts in rats.

Author information

1
Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, Tochigi, University of Tokyo, Tokyo, Japan.

Abstract

AIM:

We determined the characteristics of transgene expression of heart grafts following ex vivo gene transfer using an adenovirus vector. Transgene expression was assessed periodically in the same animals by a non-invasive bioimaging system.

METHODS:

Rat heterotopic heart transplantation was performed in a syngenic combination. We infused 1 x 10(9) plaque-forming units of adenovirus vectors containing firefly luciferase gene into the heart graft via the coronary artery, with preservation at 4 degrees C and transplanted into the cervix of the recipient. Transgene expression was periodically visualized and quantified by a noninvasive bioimaging system without sacrificing experimental animals.

RESULTS:

Transgene expression in the graft peaked at day 7 and then fell gradually. Transgene expression was also observed in the recipient liver.

CONCLUSIONS:

We have determined the time course of transgene expression in the heart graft. This constitutes important information about ex vivo gene therapy for heart grafts.

[Indexed for MEDLINE]

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