Format

Send to

Choose Destination
Cytokine. 2005 Apr 21;30(2):86-92.

Interferon-gamma associated cytokines and chemokines produced by spleen cells from Brucella-immune mice.

Author information

1
Department of Bacterial Diseases, Walter Reed Army Institute of Research, Bldg 503, Room 3E10, Robert Grant Avenue, Silver Spring, MD 20910, USA. chrysanthi.paranavitana@na.amedd.army.mil

Abstract

It is known that interferon (IFN)-gamma plays a critical role in protection against brucellosis. In this study we have investigated several cytokines and chemokines that are associated with IFN-gamma for potential in vitro correlates of protection. We cultured spleen cells in vitro from mice immunized orally with a live, attenuated Brucella melitensis vaccine candidate (WR201) and stimulated these cells with a lysate of B. melitensis. Differential gene expression of several cytokines and chemokines in stimulated spleen cells was analysed by real-time PCR, and secreted proteins were determined by ELISA. Immunized mice produced higher levels of both protein and gene transcripts for IFN-gamma, interleukin (IL)-2, IL-18 and MIP1-alpha. Immunized mice also had elevated gene expression levels for IL12-p40, IL23-p19, IP-10, MIG and MCP-1 when compared to normal mice. In this study we have identified new cytokines and chemokines as potential immune correlates in responses to protection in Brucella-vaccinated mice.

PMID:
15804600
DOI:
10.1016/j.cyto.2004.12.009
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center