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Ophthalmologica. 2005 Mar-Apr;219(2):93-6.

Failure to detect hepatitis B virus in vitreous by polymerase chain reaction.

Author information

1
Department of Ophthalmology, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey. drcaras@superonline.com

Abstract

PURPOSE:

To assay the vitreous of asymptomatic hepatitis B virus (HBV) carriers for the presence of HBV DNA using polymerase chain reaction (PCR).

METHODS:

Vitreous and serum specimens were collected from 13 carriers of HBV. The presence of HBV DNA was investigated by using PCR and Digene's Hybrid Capture System. The presence of hepatitis B surface antigen (HBsAg) in vitreous was also investigated by using the enzyme immunoassay (EIA).

RESULTS:

The serum was positive for HBV DNA in3 of the 13 asymptomatic carriers using PCR. Vitreous samples of all the patients, including 3 patients who were positive for HBV DNA in serum, were negative for HBV DNA with PCR and were negative for HBsAg with EIA.

CONCLUSION:

There is no evidence of HBV in the vitreous of asymptomatic HBV carriers.

PMID:
15802933
DOI:
10.1159/000083267
[Indexed for MEDLINE]

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