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J Biol Chem. 2005 Jun 3;280(22):21137-43. Epub 2005 Mar 30.

Concerted auto-regulation in yeast endosomal t-SNAREs.

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Department of Physiology and Biophysics, Columbia University Medical Center, New York, New York 10032, USA.


In yeast, the assembly of the target (t)-SNAREs [Tlg2p/Tlg1p,Vti1p] and [Pep12p/Tlg1p,Vti1p] with the vesicular (v)-SNARE Snc2p promotes endocytic fusion. Here, selected mutations and truncations of SNARE proteins were tested in an in vitro fusion assay to identify potential regulatory regions in these proteins, and two distinct regions were found. The first is represented by the combined effect of the three t-SNARE N-terminal regions and the second is located within the Tlg1p SNARE motif. These internal controls provide a potential mechanism to enable SNARE-dependent fusion to be regulated.

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