Format

Send to

Choose Destination
FEBS Lett. 2005 Mar 28;579(9):1923-9.

Regulation of divalent metal transporter expression in human intestinal epithelial cells following exposure to non-haem iron.

Author information

1
School of Biomedical and Molecular Sciences, University of Surrey, Guildford, UK.

Abstract

A number of regulatory factors including dietary iron levels can dramatically alter the expression of the intestinal iron transporter DMT1. Here we show that Caco-2 cells exposed to iron for 4h exhibited a significant decrease in plasma membrane DMT1 protein, though total cellular DMT1 levels were unaltered. Following biotinylation of cell surface proteins, there was a significant increase in intracellular biotin-labelled DMT1 in iron-exposed cells. Furthermore, iron-treatment increased levels of DMT1 co-localised with LAMP1, suggesting that the initial response of intestinal epithelial cells to iron involves internalisation and targeting of DMT1 transporter protein towards a late endosomal/lysosomal compartment.

PMID:
15792797
DOI:
10.1016/j.febslet.2005.02.035
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center