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Phys Rev Lett. 2005 Mar 18;94(10):108101. Epub 2005 Mar 15.

Short-range spectroscopic ruler based on a single-molecule optical switch.

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1
Division of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts 02138, USA.

Abstract

We demonstrate a novel all-optical switch consisting of two molecules: a primary fluorophore (Cy5) that can be switched between a fluorescent and a dark state by light of different wavelengths, and a secondary chromophore (Cy3) that facilitates switching. The interaction between the two molecules exhibits a distance dependence much steeper than that of conventional Fo rster resonance energy transfer. This enables the switch to act as a ruler with the capability to probe distances difficult to access by other spectroscopic methods, thus presenting a new tool for the study of biomolecules at the single-molecule level.

PMID:
15783528
PMCID:
PMC2652517
DOI:
10.1103/PhysRevLett.94.108101
[Indexed for MEDLINE]
Free PMC Article
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