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Nat Methods. 2004 Oct;1(1):27-9.

A rapid method for determining protein kinase phosphorylation specificity.

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1
Division of Signal Transduction, Harvard Medical School, 330 Brookline Avenue, Boston, Massachusetts 02215, USA.

Abstract

Selection of target substrates by protein kinases is strongly influenced by the amino acid sequence surrounding the phosphoacceptor site. Identification of the preferred peptide phosphorylation motif for a given kinase permits the production of efficient peptide substrates and greatly simplifies the mapping of phosphorylation sites in protein substrates. Here we describe a combinatorial peptide library method that allows rapid generation of phosphorylation motifs for serine/threonine kinases.

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PMID:
15782149
DOI:
10.1038/nmeth708
[Indexed for MEDLINE]

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