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Mol Ther. 2005 Apr;11(4):523-30.

Delivery of MDR1 small interfering RNA by self-complementary recombinant adeno-associated virus vector.

Author information

1
Department of Pharmacology, University of North Carolina, 1017 Mary Ellen Jones, CB# 7365, Chapel Hill, NC 27599-7365, USA.

Abstract

Small interfering RNAs (siRNAs) are potentially powerful tools for therapeutic gene regulation. DNA cassettes encoding RNA polymerase III promoter-driven hairpin siRNAs allow long-term expression of siRNA in targeted cells. A variety of viral vectors have been used to deliver such cassettes to cells. Here we report on the development and use of a self-complementary recombinant adeno-associated virus (scAAV) vector for siRNA delivery into mammalian cells. We demonstrate that this modified vector efficiently delivers siRNA into multidrug-resistant human breast and oral cancer cells and suppresses MDR1 gene expression. This results in rapid, profound, and durable reduction in the expression of the P-glycoprotein multidrug transporter and a substantial reversion of the drug-resistant phenotype. This research suggests that scAAV-based vectors can be very effective agents for efficient delivery of therapeutic siRNA.

PMID:
15771955
PMCID:
PMC1361307
DOI:
10.1016/j.ymthe.2004.12.019
[Indexed for MEDLINE]
Free PMC Article

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