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Neuroreport. 2005 Apr 4;16(5):425-9.

A neuronal cell line that does not express either prion or doppel proteins.

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Ilsong Institute of Life Science, College of Medicine, Hallym University, Ilsong Building, Anyang, Kyonggi-do 431-060, South Korea.


Prions have been extensively studied since they represent a new class of infectious agents, the pathogenic prion protein (PrPSc). However, a central question on the physiological function of the normal prion protein (PrPC) remains unresolved. A cell model which was previously established from Rikn mice (PrP-/-) remains problematic because of its ectopic expression of the doppel (Dpl) which may have a neurotoxic effect. Here we established neuronal cell lines from Z├╝rich I (PrP-/-) which do not express Dpl protein and ICR mice (PrP+/+) by transfecting with plasmid encoding for the large T antigen of SV40. The transformed cells have shown neuronal characteristics and, thus, these cell lines may provide a useful model to explore the function of neuronal PrPC.

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