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World J Gastroenterol. 2005 Mar 7;11(9):1283-6.

Prognostic values of tumor endothelial markers in patients with colorectal cancer.

Author information

1
Metastasis and Angiogenesis Research Group Department of Surgery, Wales College of Medicine University of Cardiff Heath Park, Cardiff CF14 4XN, United Kingdom. rmali@cf.ac.uk

Abstract

AIM:

Tumor endothelial markers (TEMs) are a newly discovered family of endothelial markers associated with tumor specific angiogenesis. This study sought to examine the levels of expression (qualitatively and quantitatively) for TEMs in human colon cancer.

METHODS:

Human colorectal cancer tissues (n = 48) and normal background tissues (n = 31) were obtained after surgery. RNA was extracted from frozen sections for gene amplification. The expression of TEMs (TEM-1 to TEM-8) was assessed using RT-PCR and their transcript levels were determined using real-time-quantitative PCR (Q-RT-PCR).

RESULTS:

TEM-1 (P = 0.01), TEM-7 (P = 0.04), TEM-7R (P = 0.03), TEM-8 (P = 0.001) significantly raised in colon cancer tissues compared with the levels detected in normal background tissues. The expressions of TEM-2 and TEM-6 were found to be not significantly different between tumor tissues and normal tissues (P > 0.05). Patients who had cancer penetrating into and through the muscularis propria of the bowel wall and developed nodal involvement (Dukes C) exhibited significantly higher levels of TEM -8 compared to patients who were node negative (P < 0.05). TEM-7 and TEM-7R showed high level of transcripts in Dukes C, but they were not statistically significant.

CONCLUSION:

The level of the expression of TEM-1, TEM-7, TEM-7R and TEM-8 (but not TEM-2 and TEM-6) were associated with both nodal involvement and disease progression, and may therefore, have a prognostic value in colorectal cancer.

PMID:
15761964
PMCID:
PMC4250673
DOI:
10.3748/wjg.v11.i9.1283
[Indexed for MEDLINE]
Free PMC Article

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