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Appl Environ Microbiol. 2005 Mar;71(3):1642-7.

Heterologous expression, purification, and characterization of a highly active xylose reductase from Neurospora crassa.

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1
Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave., Urbana, IL 61801, USA.

Abstract

A xylose reductase (XR) gene was identified from the Neurospora crassa whole-genome sequence, expressed heterologously in Escherichia coli, and purified as a His6-tagged fusion in high yield. This enzyme is one of the most active XRs thus far characterized and may be used for the in vitro production of xylitol.

PMID:
15746370
PMCID:
PMC1065158
DOI:
10.1128/AEM.71.3.1642-1647.2005
[Indexed for MEDLINE]
Free PMC Article
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