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J Bacteriol. 2005 Mar;187(6):2215-7.

Kinetic characterization of the glycosyltransferase module of Staphylococcus aureus PBP2.

Author information

1
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA.

Abstract

We report the heterologous overexpression and purification of Staphylococcus aureus PBP2 and demonstrate efficient glycan polymerization from lipid II in vitro. S. aureus PBP2 is the first purified gram-positive class A penicillin-binding protein to show good transglycosylase activity. This enables further studies on this important class of enzymes.

PMID:
15743972
PMCID:
PMC1064046
DOI:
10.1128/JB.187.6.2215-2217.2005
[Indexed for MEDLINE]
Free PMC Article

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